"When he came around second [base], I saw him grab his [left] leg," Trembley said. "And so when he got to third, [third-base coach Juan Samuel] asked him how he was. He said it pinched a little bit, so I took him out of the game."
Pie, who was replaced on the bases and in the outfield by Matt Angle, did not appear to be immediately hurt. Pie waited until two pitches had been thrown to the following batter, Robert Andino, before retreating to the visitors' dugout, and he said his conversation with Samuel was about erring on the cautious side.
"Juan wanted to talk to me to tell me that this is the beginning of the season, it's Spring Training," Pie said. "I'm OK now; I do my job, so [they] took me out of the game."
While Pie walked off on his own, he did appear to be favoring his right leg, which is a haunting sight for O's fans. Pie was plagued by left hamstring issues all year and was shut down for the season after straining his left quadriceps in September.
Following Thursday's game, Trembley said he wasn't worried about Pie, noting that the outfielder told him he could have stayed in if necessary.
"I told him that, obviously, he wasn't scheduled to play [Friday]," Trembley said. "He's scheduled to play Saturday in Lakeland [against the Tigers]. I think he'll be able to do that."
Pie left without any ice, wrapping or a noticeable limp, and said his leg just felt like it had been overstretched when he was running.
"It's not tight, it's stretched a little bit," Pie said. "But I'm OK."
Starting in center field, Pie homered off Rays right-hander Jeff Niemann to open the game at Charlotte Sports Park and tripled into the left-center-field gap in his second at-bat.
Despite an injury-plagued '09 campaign, a noticeably more extroverted Pie arrived in camp healthy and ready to go. He is expected to spend the season in a platoon in left field with Nolan Reimold, and Pie will figure to get some at-bats as the designated hitter to keep his legs fresh.
A former top prospect, Pie began last year as Baltimore's starting left fielder, but he lost his starting job after struggling to hit. The O's replaced Pie with Reimold, and Pie didn't begin to emerge again until center fielder Adam Jones went on the disabled list. Pie batted .333 in 23 August games and batted .290 overall after the All-Star break to salvage his season.
Brittany Ghiroli is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.