Jones was diagnosed with a mild strain and is listed as day-to-day.
Jones, who had homered twice on Tuesday night, appeared to grimace at points during his third at-bat. The youngster wound up singling through second base to tie up Baltimore's game against Tampa Bay, and then the Orioles elected to use Felix Pie as a pinch-runner and a defensive replacement.
Pie had left Tuesday's game early with a stomach illness, but further testing sanctioned his use Wednesday night. Jones had missed a game late in April with a similar hamstring injury but played without pain in recent days. Jones has batted safely in 16 of his last 18 games and leads the Orioles with eight home runs.
Spencer Fordin is a reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.