Joe Mahoney was called up from Triple-A Norfolk to take Betemit's place on the active roster.
Manager Buck Showalter said Betemit would receive an injection on Thursday night and then travel to the team's Spring Training facility in Sarasota, Fla., on Friday morning. Betemit is expected to take a week to recover before potentially returning to baseball activities.
Because Betemit has not played since Saturday, he will be eligible to come off the DL on Aug. 27.
"We have high hopes that he'll be a player for us on the 27th," Showalter said.
Betemit's sore wrist had been creating issues when he tried to swing during the past couple of days, leading the team to conduct an MRI on Thursday morning.
Prior to the injury, Betemit had been platooning at first base with Mark Reynolds. Betemit is hitting .306 against righties and .143 against lefties this season.
Mahoney started at first base and was scheduled to hit fifth for Norfolk on Thursday afternoon before being pulled in the second inning.
Mahoney caught a flight to Baltimore to try to avoid traffic and was expected to arrive around 4:40 p.m. ET, which would have allowed him to participate in the final round of batting practice.
Showalter said Mahoney's status with the team is day to day, depending on the state of the Orioles' bullpen.
"We'll see what our needs are after tonight, and after tomorrow, and after the next day," Showalter said. "Joe has done well down there, though."
Mahoney has played 118 games for Norfolk this season, hitting .263 with nine home runs and 53 RBIs.